RELATED BOOKS
The Proteomics Protocols Handbook
Language: en
Pages: 988
Authors: John M. Walker
Categories: Science
Type: BOOK - Published: 2007-10-09 - Publisher: Springer Science & Business Media

Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. All laboratory-based protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
The Protein Protocols Handbook
Language: en
Pages: 1991
Authors: John M. Walker
Categories: Science
Type: BOOK - Published: 2009 - Publisher:

Since the publication of the bestselling second edition of John Walker's widely acclaimed Protein Protocols Handbook, there have been continual methodological developments in the field of protein chemistry. This greatly enhanced third edition introduces 57 critically important new chapters, as well as significantly updating the previous edition's tried-and-true methods. Although the timely new chapters are spread throughout all of the book, the vital section on post-translational modifications has been expanded most to reflect the increasing importance of these modifications in the understanding of protein function. Each readily reproducible method follows the highly praised format of the Methods in Molecular Biology series, offering a concise summary of its basic theory, a complete materials list, a step-by-step protocol for its successful execution, and extensive notes on avoiding pitfalls, or on modifying the method to function within your own experimental circumstances. The expert authors of each chapter have demonstrated a hands-on mastery of the methods described, fine-tuned here for optimal productivity.Comprehensive, cutting-edge, and highly practical, The Protein Protocols Handbook, Third Edition is today's indispensable benchtop manual and guide, not only for all those new to the protein chemistry laboratory, but also for those established workers seeking to broaden their armamentarium of techniques in the urgent search for rapid and robust results.
Guide to Protein Purification
Language: en
Pages: 900
Authors: Richard R Burgess, Murray P. Deutscher
Categories: Science
Type: BOOK - Published: 2009-11-03 - Publisher: Academic Press

The 2e of this classic Guide to Protein Purification provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition’s publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology Assembles chapters on both common and less common relevant techniques Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process
Biomedical Photonics Handbook, Second Edition
Language: en
Pages: 889
Authors: Tuan Vo-Dinh
Categories: Medical
Type: BOOK - Published: 2014-07-29 - Publisher: CRC Press

Shaped by Quantum Theory, Technology, and the Genomics Revolution The integration of photonics, electronics, biomaterials, and nanotechnology holds great promise for the future of medicine. This topic has recently experienced an explosive growth due to the noninvasive or minimally invasive nature and the cost-effectiveness of photonic modalities in medical diagnostics and therapy. The second edition of the Biomedical Photonics Handbook presents fundamental developments as well as important applications of biomedical photonics of interest to scientists, engineers, manufacturers, teachers, students, and clinical providers. The second volume, Biomedical Diagnostics, focuses on biomedical diagnostic technologies and their applications from the bench to the bedside. Represents the Collective Work of over 150 Scientists, Engineers, and Clinicians Designed to display the most recent advances in instrumentation and methods, as well as clinical applications in important areas of biomedical photonics to a broad audience, this three-volume handbook provides an inclusive forum that serves as an authoritative reference source for a broad audience involved in the research, teaching, learning, and practice of medical technologies. What’s New in This Edition: A wide variety of photonic biochemical sensing technologies have already been developed for clinical monitoring of physiological parameters, such as blood pressure, blood chemistry, pH, temperature, and the presence of pathological organisms or biochemical species of clinical importance. Advanced photonic detection technologies integrating the latest knowledge of genomics, proteomics and metabolomics allow sensing of early disease state biomarkers, thus revolutionizing the medicine of the future. Nanobiotechnology has opened new possibilities for detection of biomarkers of disease, imaging single molecules and in situ diagnostics at the single cell level. In addition to these state-of-the art advancements, the second edition contains new topics and chapters including: • Fiber Optic Probe Design • Laser and Optical Radiation Safety • Photothermal Detection • Multidimensional Fluorescence Imaging • Surface Plasmon Resonance Imaging • Molecular Contrast Optical Coherence Tomography • Multiscale Photoacoustics • Polarized Light for Medical Diagnostics • Quantitative Diffuse Reflectance Imaging • Interferometric Light Scattering • Nonlinear Interferometric Vibrational Imaging • Multimodality Theranostics Nanoplatforms • Nanoscintillator-Based Therapy • SERS Molecular Sentinel Nanoprobes • Plasmonic Coupling Interference Nanoprobes Comprised of three books: Volume I: Fundamentals, Devices, and Techniques; Volume II: Biomedical Diagnostics; and Volume III: Therapeutics and Advanced Biophotonics, this second edition contains eight sections, and provides introductory material in each chapter. It also includes an overview of the topic, an extensive collection of spectroscopic data, and lists of references for further reading.
Analysis of Biological Networks
Language: en
Pages: 368
Authors: Björn H. Junker, Falk Schreiber
Categories: Computers
Type: BOOK - Published: 2011-09-20 - Publisher: John Wiley & Sons

An introduction to biological networks and methods for theiranalysis Analysis of Biological Networks is the first book of itskind to provide readers with a comprehensive introduction to thestructural analysis of biological networks at the interface ofbiology and computer science. The book begins with a brief overviewof biological networks and graph theory/graph algorithms and goeson to explore: global network properties, network centralities,network motifs, network clustering, Petri nets, signal transductionand gene regulation networks, protein interaction networks,metabolic networks, phylogenetic networks, ecological networks, andcorrelation networks. Analysis of Biological Networks is a self-containedintroduction to this important research topic, assumes no expertknowledge in computer science or biology, and is accessible toprofessionals and students alike. Each chapter concludes with asummary of main points and with exercises for readers to test theirunderstanding of the material presented. Additionally, an FTP sitewith links to author-provided data for the book is available fordeeper study. This book is suitable as a resource for researchers in computerscience, biology, bioinformatics, advanced biochemistry, and thelife sciences, and also serves as an ideal reference text forgraduate-level courses in bioinformatics and biologicalresearch.
Microbial Proteomics: Development in Technologies and Applications
Language: en
Pages: 236
Authors: Divakar Sharma
Categories: Science
Type: BOOK - Published: 2020-12-31 - Publisher: Bentham Science Publishers

This volume brings current knowledge of proteomics technologies and related developments with special reference to diseases caused by microbes. The editor has compiled chapters written by expert academicians which distill the information about useful methods in microbial proteomics for the benefit of readers. Chapters cover several methods used to investigate the microbial proteome and special topics such as antimicrobial drug resistance mechanisms, biomarker developments, post translational modifications. Key Features: -overview of several biochemical methods in proteomics -full-color, high quality images of the most frequent technologies and applications -concise, well organized, and didactic format -updates in basic applied information -bibliographic references -information on proteomics for tuberculosis treatment This reference work is intended for researchers seeking information on laboratory techniques applied in proteomics research and microbiology.
Handbook of Dairy Foods Analysis
Language: en
Pages: 918
Authors: Leo M.L. Nollet, Fidel Toldra
Categories: Technology & Engineering
Type: BOOK - Published: 2009-11-04 - Publisher: CRC Press

Dairy foods account for a large portion of the Western diet, but due to the potential diversity of their sources, this food group often poses a challenge for food scientists and their research efforts. Bringing together the foremost minds in dairy research, Handbook of Dairy Foods Analysis compiles the top dairy analysis techniques and methodologies from around the world into one, well-organized volume. Co-Edited by Fidel Toldra - Recipient of the 2010 Distinguished Research Award from the American Meat Science Association Exceptionally comprehensive both in its detailing of methods and the range of products covered, this handbook includes tools for analyzing chemical and biochemical compounds and also bioactive peptides, prebiotics, and probiotics. It describes noninvasive chemical and physical sensors and starter cultures used in quality control. Covers the Gamut of Dairy Analysis Techniques The book discusses current methods for the detection of microorganisms, allergens, and other adulterations, including those of environmental origin or introduced during processing. Other methodologies used to evaluate color, texture, and flavor are also discussed. Written by an International Panel of Distinguished Contributors Under the editorial guidance of renowned authorities, Leo M.L. Nollet and Fidel Toldrá, this handbook is one of the few references that is completely devoted to dairy food analysis – a extremely valuable reference for those in the dairy research, processing, and manufacturing industries.
Biomolecular and Bioanalytical Techniques
Language: en
Pages: 576
Authors: Vasudevan Ramesh
Categories: Science
Type: BOOK - Published: 2019-03-08 - Publisher: John Wiley & Sons

An essential guide to biomolecular and bioanalytical techniques and their applications Biomolecular and Bioanalytical Techniques offers an introduction to, and a basic understanding of, a wide range of biophysical techniques. The text takes an interdisciplinary approach with contributions from a panel of distinguished experts. With a focus on research, the text comprehensively covers a broad selection of topics drawn from contemporary research in the fields of chemistry and biology. Each of the internationally reputed authors has contributed a single chapter on a specific technique. The chapters cover the specific technique’s background, theory, principles, technique, methodology, protocol and applications. The text explores the use of a variety of analytical tools to characterise biological samples. The contributors explain how to identify and quantify biochemically important molecules, including small molecules as well as biological macromolecules such as enzymes, antibodies, proteins, peptides and nucleic acids. This book is filled with essential knowledge and explores the skills needed to carry out the research and development roles in academic and industrial laboratories. A technique-focused book that bridges the gap between an introductory text and a book on advanced research methods Provides the necessary background and skills needed to advance the research methods Features a structured approach within each chapter Demonstrates an interdisciplinary approach that serves to develop independent thinking Written for students in chemistry, biological, medical, pharmaceutical, forensic and biophysical sciences, Biomolecular and Bioanalytical Techniques is an in-depth review of the most current biomolecular and bioanalytical techniques in the field.
Proteomics of Biological Systems
Language: en
Pages: 376
Authors: Bryan M. Ham
Categories: Science
Type: BOOK - Published: 2011-09-26 - Publisher: John Wiley & Sons

Phosphorylation is the addition of a phosphate (PO4) group to a protein or other organic molecule. Phosphorylation activates or deactivates many protein enzymes, causing or preventing the mechanisms of diseases such as cancer and diabetes. This book shows how to use mass spectrometry to determine whether or not a protein has been correctly modified by the addition of a phosphate group. It also provides a combination of detailed, step-by-step methodology for phosphoproteomic sample preparation, mass spectral instrumental analysis, and data interpretation approaches. Furthermore, it includes the use of bioinformatic Internet tools such as the Blast2GO gene ontology (GO) tool, used to help understand and interpret complex data collected in these studies.
Computational Methods for Mass Spectrometry Proteomics
Language: en
Pages: 296
Authors: Ingvar Eidhammer, Kristian Flikka, Lennart Martens, Svein-Ole Mikalsen
Categories: Medical
Type: BOOK - Published: 2008-02-28 - Publisher: John Wiley & Sons

Proteomics is the study of the subsets of proteins present in different parts of an organism and how they change with time and varying conditions. Mass spectrometry is the leading technology used in proteomics, and the field relies heavily on bioinformatics to process and analyze the acquired data. Since recent years have seen tremendous developments in instrumentation and proteomics-related bioinformatics, there is clearly a need for a solid introduction to the crossroads where proteomics and bioinformatics meet. Computational Methods for Mass Spectrometry Proteomics describes the different instruments and methodologies used in proteomics in a unified manner. The authors put an emphasis on the computational methods for the different phases of a proteomics analysis, but the underlying principles in protein chemistry and instrument technology are also described. The book is illustrated by a number of figures and examples, and contains exercises for the reader. Written in an accessible yet rigorous style, it is a valuable reference for both informaticians and biologists. Computational Methods for Mass Spectrometry Proteomics is suited for advanced undergraduate and graduate students of bioinformatics and molecular biology with an interest in proteomics. It also provides a good introduction and reference source for researchers new to proteomics, and for people who come into more peripheral contact with the field.
Proteome Research
Language: en
Pages: 239
Authors: M.R. Wilkins, R.D. Appel, K.L. Williams, D.F. Hochstrasser
Categories: Science
Type: BOOK - Published: 2008-02-05 - Publisher: Springer Science & Business Media

Proteomics is a multifaceted, interdisciplinary field which studies the complexity and dynamics of proteins in biological systems. It combines powerful separation and analytical technology with advanced informatics to understand the function of proteins in the cell and in the body. This book provides a clear conceptual description of each facet of proteomics, describes recent advances in technology and thinking in each area, and provides details of how these have been applied to a variety of biological problems. It is written by expert practitioners in the field, from industry, research institutions, and the clinic. It provides junior and experienced researchers with an invaluable proteomic reference, and gives fascinating glimpses of the future of this dynamic field.
Proteomics and Peptidomics
Language: en
Pages: 632
Authors: Gyorgy Marko-Varga
Categories: Medical
Type: BOOK - Published: 2005 - Publisher: Elsevier

Proteomics and peptidomics is the detailed understanding of the role that proteins and peptides play in health and disease and is a necessary compliment to genetic analysis. The functional expression analysis of both proteins and peptides plays a central role in modern drug discovery as well as drug development, and is also a key research area in systems biology.Proteomics and Peptidomics captures the width as well as the depth within the area and exemplifies the variety as well as the traditional basis of analytical chemistry that is needed in order to move forward in expression analysis studies. As a fast emerging field, it gives and overview of parts within the field combined with highly specialized and dedicated topics that are intended to compliment each other.